Cisplatin-induced changes in the number of argyrophilic nucleolar granules in cultured chick dorsal root ganglion neurons
✍ Scribed by R. Tureček; V. Mandys; P.R. Bär; Z. Likovský
- Publisher
- John Wiley and Sons
- Year
- 1996
- Tongue
- English
- Weight
- 448 KB
- Volume
- 18
- Category
- Article
- ISSN
- 0893-6609
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✦ Synopsis
Changes in the number of argyrophilic nucleolar granules (ANGs) caused by cisplatin treatment were studied in sensory neurons isolated from dorsal root ganglia of 12day-old chick embryos. The number of ANGs changed during culture in untreated cultures. The mean number of ANGs per cell was 10.6 f 4.6 at 24 h. A dramatic increase of ANGs (mean number 32.8 f 6.7) was observed after 48 h in vitro, followed by a slight decrease to 26.8 f 0.3 at 72 h. Cisplatin treatment caused a significant decrease of the number of ANGs per neuron depending on the concentration of the drug in culture medium as well as on the exposure time. The profile of changes of ANGs within the time was at low concentrations of cisplatin (1 and 2.5 pg/ml) identical with untreated cultures. The mean number of ANGs was Unchanged in the cells exposed for 24 h only and reduced in cultures treated for 48 and 72 h. At higher concentrations of cisplatin (from 5 to 20 pglml), a prominent decrease of the mean number of ANGs was observed in all cultures in all t h e intervals. These findings provide evidence that cisplatin influences the nucleolar organizer regions of dorsal root ganglia neurons. Thus, nucleolar RNA and protein synthesis can be reduced. This alteration of cell metabolism probably contributes to the development of peripheral sensory neuropathy .