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Operon fusions in the nitrate reductase operon and study of the control gene nir R in Escherichia coli

✍ Scribed by Chippaux, Marc ;Bonnefoy-Orth, Violaine ;Ratouchniak, Jeannine ;Pascal, Marie-Claire

Publisher: Springer
Year: 1981
Tongue: English
Weight: 389 KB
Volume: 182
Category: Article
ISSN: 0026-8925
DOI: 10.1007/bf00293938

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✦ Synopsis

Strains carrying operon fusions between the promotor of the chl I gene and the lac structural genes were constructed. From these strains in which the expression of the lac genes is under the control of both nitrate and oxygen, spontaneous regulatory mutants were selected: (i) mutants which synthesize beta-galactosidase constitutively in anaerobiosis; (ii) mutants in which beta-galactosidase synthesis is no longer repressed by oxygen. Introduction of the nir R mutated allele into strains carrying these fusions resulted in the total loss of beta-galactosidase synthesis, confirming that nir R is a regulatory gene controlling the expression of the biosynthesis of the nitrate reductase.

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