Strains carrying operon fusions between the promotor of the chl I gene and the lac structural genes were constructed. From these strains in which the expression of the lac genes is under the control of both nitrate and oxygen, spontaneous regulatory mutants were selected: (i) mutants which synthesiz
Autoregulation of the nar operon encoding nitrate reductase in Escherichia coli
β Scribed by Bonnefoy, Violaine ;Pascal, Marie-Claire ;Ratouchniak, Jeanine ;Chippaux, Marc
- Publisher
- Springer
- Year
- 1986
- Tongue
- English
- Weight
- 469 KB
- Volume
- 204
- Category
- Article
- ISSN
- 0026-8925
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β¦ Synopsis
Nitrate reductase is demonstrated to exert an autogenous control on its own synthesis. This effect requires the participation of the molybdenum cofactor. Use of strains in which the control region of the nar operon is mutated reveals two loci in this region: one, affected in strain LCB94, is common to both autoregulation and induction by nitrate while the other, mutated in strain LCB188, is specific for the induction by nitrate. It is proposed that the autogenous control prevents the unnecessary accumulation of the nitrate reductase subunits in the cytoplasm.
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The nar promoter of Escherichia coli, which is maximally induced under anaerobic conditions in the presence of nitrate, was characterized to see whether the nar promoter cloned onto pBR322 can be used as an inducible promoter. To increase the expression level, the nar promoter was expressed in β¬. co
The nar promoter of Escherichia coli is maximally induced under anaerobic or microaerobic conditions in the presence of nitrate. We previously demonstrated in batch experiments that the intact nar promoter of E. coli cloned into a pBR322-based plasmid serves as a high-level expression system in a na
## Abstract Nitrate reductase was investigated in extracts from cells of a chlorateβresistant mutant strain of __E. coli__ which grew anaerobically on nitrate as the sole source of nitrogen. The nitrate reductase was of particulate nature and reduced chlorate like the nitrate reductase from the wil