Open-loop control of specific growth rate in fed-batch cultures of recombinantE.coli
โ Scribed by M. E. Gregory; C. Turner
- Publisher
- Springer-Verlag
- Year
- 1993
- Tongue
- English
- Weight
- 418 KB
- Volume
- 7
- Category
- Article
- ISSN
- 0951-208X
No coin nor oath required. For personal study only.
โฆ Synopsis
A computer-based algorithm was used for the open-loop control of specific growth rate in fedbatch cultures of recombinant E.coli.. The control of nutrient feed rate to an exponential trajectory resulted in growth of the culture at a constant specific growth rate. Stable specific growth rates between 0.08 and 0.4 h-1 were achieved.
๐ SIMILAR VOLUMES
This paper develops a practical and useful computer control scheme to control the specific growth rate as accurately as possible by measuring the released protons in anaerobic alcohol fermentation. In the. case of current study, most of the released protons are due to the uptake of cationic ammoniu
A feb-batch operation for the production of bovine somatotropin (bST) under the control of tryptophan promoter in Escherichia coli was investigated. The plasmid used contains a two-cistron system and altered codon usage for higher expression of bST. Specific growth rate is an important parameter in