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One-step purification of histidine-tagged profilin with high purity and yield by using metal precipitation

✍ Scribed by Mojtaba Sankian; Mitra Yousefi; Nazanin Pazouki; Abdolreza Varasteh


Book ID
111718167
Publisher
Portland Press
Year
2007
Tongue
English
Weight
230 KB
Volume
47
Category
Article
ISSN
0885-4513

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## Abstract Metal chelate affinity precipitation (MCAP) has been successfully developed as a simple purification process for proteins that have affinity for metal ions. The present lack of widespread applications for this technique as compared to immobilized metal affinity chromatography (IMAC) may