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[Methods in Biotechnology™] Immobilization of Enzymes and Cells Volume 22 || One-Step Purification, Immobilization, and Stabilization of Poly-Histidine-Tagged Enzymes Using Metal Chelate-Epoxy Supports

✍ Scribed by Guisan, Jose M.

Book ID
120517585
Publisher
Humana Press
Year
2006
Weight
203 KB
Category
Article
ISBN
1597450537

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## Abstract Epoxy supports covalently immobilize proteins following a two‐step mechanism; that is, the protein is physically adsorbed and then the covalent reaction takes place. This mechanism has been exploited to combine the selectivity of metal chelate affinity chromatography with the covalent i