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One-step immobilization and purification of his-tagged enzyme using poly(2-acetamidoacrylic acid) hydrogel

โœ Scribed by Eun-Ju Ha, Kyeong Kyu Kim, Hyung Soon Park, Sun-Gu Lee, Jang-Oo Lee, Seong Soo A. An, Hyun-jong Paik

Book ID
118302572
Publisher
The Polymer Society of Korea
Year
2012
Tongue
English
Weight
582 KB
Volume
21
Category
Article
ISSN
1598-5032

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One-step purification, covalent immobili
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## Abstract Epoxy supports covalently immobilize proteins following a twoโ€step mechanism; that is, the protein is physically adsorbed and then the covalent reaction takes place. This mechanism has been exploited to combine the selectivity of metal chelate affinity chromatography with the covalent i