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One-Step Purification, Covalent Immobilization, and Additional Stabilization of a Thermophilic Poly-His-Tagged β-Galactosidase from Thermus sp. Strain T2 by using Novel Heterofunctional Chelate−Epoxy Sepabeads

✍ Scribed by Pessela, Benevides C. C.; Mateo, Cesar; Carrascosa, Alfonso V.; Vian, Alejandro; García, José L.; Rivas, German; Alfonso, Carlos; Guisan, José M.; Fernández-Lafuente, Roberto


Book ID
119995134
Publisher
American Chemical Society
Year
2003
Tongue
English
Weight
188 KB
Volume
4
Category
Article
ISSN
1525-7797

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One-step purification, covalent immobili
✍ Cesar Mateo; Gloria Fernández-Lorente; Estrella Cortés; José L. Garcia; Roberto 📂 Article 📅 2001 🏛 John Wiley and Sons 🌐 English ⚖ 482 KB

## Abstract Epoxy supports covalently immobilize proteins following a two‐step mechanism; that is, the protein is physically adsorbed and then the covalent reaction takes place. This mechanism has been exploited to combine the selectivity of metal chelate affinity chromatography with the covalent i