๐”– Bobbio Scriptorium
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One-carbon metabolism and pyridoxal 5'-phosphate biosynthesis

โœ Scribed by Dempsey, Walter B.


Book ID
123034769
Publisher
Elsevier Science
Year
1970
Tongue
English
Weight
110 KB
Volume
222
Category
Article
ISSN
0304-4165

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A new and very sensitive fiuorometric method for the determination of pyridoxal and pyridoxal 5'-phosphate is reported. The specificity is based on the reductive amination of pyridoxal and its Y-phosphate with methyl anthranilate and sodium cyanoborohydride at pH 4.5 to 5.0. Separation of the highly

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Markedly increased circulating concentrations of pyridoxal-5'-phosphate (PLP) were found in each of 14 patients representing all clinical forms of bypophosphatasia, an inborn error characterized by deficient activity of the tissue-nonspecific (bone/liver/kidney) isoenzyme of alkaline phosphatase (AP