On the stretching of DNA in the reptation theories of gel electrophoresis
โ Scribed by Gary W. Slater; Jean Rousseau; Jaan Noolandi
- Publisher
- Wiley (John Wiley & Sons)
- Year
- 1987
- Tongue
- English
- Weight
- 557 KB
- Volume
- 26
- Category
- Article
- ISSN
- 0006-3525
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โฆ Synopsis
The reptation dynamics of DNA chains in gel electrophoresis is discussed. The differences between the Lumpkin, Dbjardin, and Zimm (LDZ), and the Slater and Noolandi (SN), biased reptation models are pointed out and studied. We show that new assumptions are necessary in order to study large-field electrophoresis, and we present preliminary results of a computer simulation of DNA gel electrophoresis.
๐ SIMILAR VOLUMES
The biased reptation model provides a good framework for interpreting the results of continuous field DNA electrophoresis experiments performed in agar-gels. Here we discuss the main features of the mobility-molecular size and mobility-gel concentration diagrams as obtained from new extensive comput
Electrophoretic mobilities of DNA molecules ranging in length from 200 to 48 502 base pairs (bp) were measured in agarose gels with concentrations T = 0.5% to 1.3% at electric fields from E = 0.71 to 5.0 V/cm. This broad data set determines a range of conditions over which the new interpolation equa