## Abstract The ability to measure ATP synthesis rates using ^31^PβNMR spectroscopy is demonstrated in the normal, ischemic, and postischemic myocardium __in vivo.__ Cardiopulmonary bypass (CBP) was employed to induce 20 min of global myocardial ischemia, and to conduct magnetization transfer measu
On the state of the magnesium ion in intact cells as observed by noninvasive 31p NMR spectroscopy
β Scribed by Raj K. Gupta
- Publisher
- John Wiley and Sons
- Year
- 2009
- Tongue
- English
- Weight
- 333 KB
- Volume
- 18
- Category
- Article
- ISSN
- 0020-7608
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## Abstract __In vivo__ ^31^P nuclear magnetic resonance (NMR) spectroscopy provides unique opportunities to study the biochemistry of an organ within the intact animal in a totally noninvasive way. We have used __in vivo__ and __in vitro__ ^31^P NMR spectroscopy to study steady state changes in th
The effects that haemoglobin-metabolite interactions have on estimates of free magnesium-ion concentration in human erythrocytes, determined by 31P NMR [Gupta, R. K. et al., J. Biol. Chem. 253, 6172-6176 (1978)], were investigated. If the metabolite-haemoglobin association constants of Berger et al.
## Abstract The measurement of free intracellular magnesium (Mg^2+^) using the ^31^P chemical shifts of ATP requires the use of appropriate calibration solutions to determine the chemicalβshift limits Ξ^ATP^~Ξ±Ξ²~ and Ξ^MgATP^~Ξ±Ξ²~. Solutions containing excess Mg^2+^ contain significant amounts of Mg~