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Error in the calibration of the mgatp chemical-shift limit: Effects on the determination of free magnesium by 31P NMR spectroscopy

✍ Scribed by Timothy J. Mosher; Gerald D. Williams; Chris Doumen; Kathryn F. Lanoue; Michael B. Smith

Publisher
John Wiley and Sons
Year
1992
Tongue
English
Weight
423 KB
Volume
24
Category
Article
ISSN
0740-3194

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✦ Synopsis

Abstract

The measurement of free intracellular magnesium (Mg^2+^) using the ^31^P chemical shifts of ATP requires the use of appropriate calibration solutions to determine the chemical‐shift limits Δ^ATP^~αβ~ and Δ^MgATP^~αβ~. Solutions containing excess Mg^2+^ contain significant amounts of Mg~2~ATP and yield positive errors in the value of Δ^MgATP^~αβ~. For physiological applications this may overestimate free intracellular Mg^2+^ by as much as 300%. This error may be minimized if appropriate mole ratios of Mg^2+^/ATP are used to calibrate Δ^MgATP^~αβ~. © 1992 Academic Press, Inc.

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