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On the stability of micro-LC columns (packed fused silica capillary columns)

✍ Scribed by De Weerdt, M. ;Dewaele, C. ;Verzele, M.

Publisher
John Wiley and Sons
Year
1987
Tongue
English
Weight
316 KB
Volume
10
Category
Article
ISSN
0935-6304

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✦ Synopsis

The life expectancy and resistance to flow, pressure shocks, solvent gradients, and bending of packed fused silica capillary columns (Micro-LC) are discussed.

2 Instrumentation

Chromatographic measurements were carried out on a Varian 5020 (Varian Associates, Walnut Creek, CA, USA) and on a Perkin Elmer LC-4(Perkin Elmer, Norwalk, CT, USA) instrument. The columns, fused silica coated with polyimide, had a length of 10 to 25 cm and an internal diameter (i.d.) of 320 pm. They were self produced or obtained from RSLlAlltech Europe (Eke, Belgium), who recently introduc-ed such columns commercially. Packing such columns is not straightforward. Special know-how, which cannot be divulged as yet, had to be developed. The packing material was mostly 5 pm ROSiL-C18-D, a spherical, octadecylated, silica based packing material from RSL/Alltech Europe. The detector was a variable wavelength UV detector (a Varian 2050 or a similar instrument from Wescan, Kontron, or Jasco) adapted to accept the Micro-LC columns. The technological aspects for converting these detectors to Micro-LC, have been detailed [4].

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