On the mechanism of chorismate mutases:clues from wild-type E. coli enzyme and asite-directed mutant related to yeast chorismate mutase

On the mechanism of chorismate mutases:

On the mechanism of chorismate mutases: Clues from wild-type E. coli enzyme and a site-directed mutant related to yeast chorismate mutase

✍ Christophe C Galopin; Sheng Zhang; David B Wilson; Bruce Ganem 📂 Article 📅 1996 🏛 Elsevier Science 🌐 French ⚖ 207 KB

Thermodynamic parameters for E. coli chorismate mutase are reported. In addition, site-directed mutagenesis studies provide a direct mechanistic link to yeast chorismate mutase, further indicating the importance of entropic restriction and hydrogen bonding in the enzymic catalysis of Claisen rearran