In the present study attempts are made to give an expression of the fatigue crack initiation life of notched elements with the consideration of overloading effects. This expression may be used to predict the fatigue crack initiation life of notched element under variable amplitude loading. Experimental work on LY12CZ alloy show that the test results of fatigue crack initiation life after tension overloading can be well fitted by the formula developed before for fatigue crack initiation life. Tension overloading increases the fatigue crack initiation threshold but has no effect on the coefficient of the resistance to fatigue crack initiation. The overloading ratio has no markable effect on crack initiation life. The increase of the crack initiation threshold results in the increase of crack initiation life, in particular, in long life range. The same results are also obtained by reanalysing some existing test results of overloading effect on crack initiation life given in literature. Consequently, the expression of the fatigue crack initiation life can be obtained by the method given in this paper. However, the overloading stress should be determined from the theoretical stress concentration factor of notched element and the maximum nominal stress in the load spectrum of elements.