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✦   LIBER   ✦

On the choice of laser dyes for use in exciting tyrosine fluorescence decays

✍ Scribed by Louis J. Libertini; Enoch W. Small

Publisher
Elsevier Science
Year
1987
Tongue
English
Weight
503 KB
Volume
163
Category
Article
ISSN
0003-2697

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✦ Synopsis

The choice of laser dyes for exciting tyrosine fluorescence using synchronously pumped cavity-dumped dye laser systems is discussed. Rhodamine 560 was found to be optimal for a system based on an argon-ion pumping laser, whereas rhodamine 575 was preferred using a frequency-doubled Nd:YAG laser. Modifications of our fluorescence decay instrument to permit rejection of multiphoton events using a microchannel plate photomultiplier are described. An example of a four-component resolution of tyrosine decays illustrates the dramatic resolution capabilities attainable. 0 1987 Academic PIES, II-C.

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