On the application of immunological techniques in geographic group studies of Atlantic sea herring,Clupea harengus II. Herring serum analysis by gel diffusion

✍ Scribed by DiCapua, Richard A.

• Publisher: John Wiley and Sons
• Year: 1969
• Tongue: English
• Weight: 975 KB
• Volume: 172
• Category: Article
• ISSN: 0022-104X
• DOI: 10.1002/jez.1401720103

✦ Synopsis

The sera of Atlantic sea herring, collected from 13 contiguous oceanic locations, were assayed by gel (double) diffusion for precipitinogen differences. A n immune serum (rabbit) against the sera of each location was required. Gel diffusion patterns characteristic of specific geographic origins of herring sera were not detected. Immune serum aliquots were therefore individually neutralized with one of a series of dilutions of herring serum from the various geographic locations prior to diffusion (the use of undiluted serum results i n complete precipitin neutralization regardless of its origin). The neutralization capacity of origin-separate precipitinogens appears to be related to the geographical distance separating the origins of immunizing and neutralizing sera. Sera of unknown origins, providing they originate from the overall area sampled, can be related to sera of specific origins by comparison of their neutralization capacities. The gel diffusion results are in agreement with previously reported immunoelectrophoretic data (DiCapua, 1966) and are consistent regardless of sex, pool size constituting the sample or collection date. The serological relationship of the five dominant clupeoid species is examined.