On the application of immunological techniques in geographic group studies of Atlantic sea herring,Clupea harengus. I. Neutralization of immune precipitins as an aid to herring serum analysis by immunoelectrophoresis

✍ Scribed by Di Capua, Richard A.

• Publisher: John Wiley and Sons
• Year: 1966
• Tongue: English
• Weight: 737 KB
• Volume: 162
• Category: Article
• ISSN: 0022-104X
• DOI: 10.1002/jez.1401620102

✦ Synopsis

The sera of Atlantic sea herring, collected from 13 contiguous oceanic locations, were analyzed by immunoelectrophoresis for precipitinogen differences. An immune serum (rabbit) against the sera from each location was required. Electtophoretic characteristics suitable for identifying the geographic origins of herring sera were not detected. Immune serum aliquots were therefore individually neutralized with undiluted or one of a series of dilutions of herring serum from each geographic location prior to analysis. The use of undiluted serum resulted in complete precipitin neutralization regardless of its origin. Equivalent dilutions of each serum resulted in varying degrees of precipitin neutralization. This capacity of origin-separate precipitinogens appears to be related to the geographical distance separating the origins of immunizing and neutralizing sera, i.e., the greater the distance the higher the concentration necessary for equivalent neutralization. Sera of unknown origins can be related to sera from specific origins by comparison a t equivalent dilutions for equivalent precipitin neutralization, providing they originate from the overall area sampled. Immunoelectrophoretic results are consistent regardless of sex, number of individuals contributing to the sample or the collection date. Herring precipitinogens are identified by comparison with the well-established mobilities of human orecinitinogens and are discussed based on the with geographic group differences.