Time-Resolved Photolabeling of Membrane
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George H. Addona; Marek A. Kloczewiak; Keith W. Miller
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Article
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1999
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Elsevier Science
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English
⚖ 69 KB
An apparatus has been developed that allows photoaffinity ligands to be crossed-linked to milligram quantities of membrane proteins with maximum attainable yield following contact times of approximately 1 ms. The apparatus consisted of three parts: a conventional rapid mixing unit, a novel freeze-qu