On peptide de novo sequencing: a new approach
β Scribed by Dr Renato Bruni; Gianluigi Gianfranceschi; Giorgio Koch
- Publisher
- John Wiley and Sons
- Year
- 2005
- Tongue
- English
- Weight
- 166 KB
- Volume
- 11
- Category
- Article
- ISSN
- 1075-2617
- DOI
- 10.1002/psc.595
No coin nor oath required. For personal study only.
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De novo peptide sequencing in an ion trap mass spectrometer coupled on-line with a capillary HPLC using 18O labeling provides a viable alternative to the method using the combination of nanospray, 18 O labeling and a quadrupole/time-of-flight mass spectrometer. Seven to sixteen amino acid residues c
Protein microanalysis usually involves the sequencing of gel-separated proteins available in very small amounts. While mass spectrometry has become the method of choice for identifying proteins in databases, in almost all laboratories 'de novo' protein sequencing is still performed by Edman degradat