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De novo peptide sequencing in an ion trap mass spectrometer with 18O labeling

✍ Scribed by Jun Qin; Christopher J. Herring; Xiaolong Zhang

Publisher: John Wiley and Sons
Year: 1998
Tongue: English
Weight: 195 KB
Volume: 12
Category: Article
ISSN: 0951-4198
DOI: 10.1002/(sici)1097-0231(19980314)12:5<209::aid-rcm141>3.0.co;2-s

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✦ Synopsis

De novo peptide sequencing in an ion trap mass spectrometer coupled on-line with a capillary HPLC using 18O labeling provides a viable alternative to the method using the combination of nanospray, 18 O labeling and a quadrupole/time-of-flight mass spectrometer. Seven to sixteen amino acid residues can be sequenced from the liquid chromatography/randem mass spectrometry (LC/MS/MS) spectra. This approach combines the benefit of capillary LC and the high sensitivity of the ion trap operated in the MS/MS mode. The wide availability of the LCQ mass spectrometer makes this approach readily adaptable to the biological mass spectrometry community.

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