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Charge derivatization of peptides to simplify their sequencing with an ion trap mass spectrometer

✍ Scribed by Maciej Adamczyk; John C. Gebler; Jiang Wu

Publisher: John Wiley and Sons
Year: 1999
Tongue: English
Weight: 118 KB
Volume: 13
Category: Article
ISSN: 0951-4198
DOI: 10.1002/(sici)1097-0231(19990730)13:14<1413::aid-rcm657>3.0.co;2-4

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✦ Synopsis

The low energy collision-induced dissociation of fixed-charge derivatives [tris(2,4,6-trimethoxyphenyl)phosphonium] of peptides was investigated using an electrospray ion trap mass spectrometer. The fixed charge directed the fragmentation pattern and generated solely N-terminal fragments with minimal internal rearrangement, regardless of the presence and position of basic amino acids in the peptide chain. Generally only b-type ions, accompanied by less intense a-type ions, were observed, depending on the collision energy. It was observed that the fixed charge controlled the fragmentation beyond typical MS/MS, and thus the capacity of the ion trap to perform multiple stage fragmentation (MS n ) was found particularly useful for obtaining the complete sequence information of the peptides.

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