𝔖 Bobbio Scriptorium
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On Penicillin-Binding Protein 1b Affinity-Labeling Reagents

✍ Scribed by Daniela Volke; Mohammed Daghish; Lothar Hennig; Matthias Findeisen; Sabine Giesa; Ramona Oehme; Peter Welzel

Book ID
102256241
Publisher
John Wiley and Sons
Year
2003
Tongue
German
Weight
274 KB
Volume
86
Category
Article
ISSN
0018-019X

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✦ Synopsis

Abstract

The synthesis of some 3‐aryl‐3‐(trifluoromethyl)3__H__‐diazirine and benzophenone‐based photoaffinity labels is reported. The photolabile group is bound to a scaffold that also accommodates functional groups to which an indicator unit (biotin) and the bioactive ligand can be attached orthogonally. To three of the labels, moenomycin was conjugated with the aim to provide tools for the identification of the moenomycin binding site within the transglycosylase domain of the enzyme PBP 1b. Some preliminary photoaffinity‐labeling experiments were carried out.

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