On-line measurements of culture fluorescence: Method and application

✍ Scribed by Walter Beyeler; Arthur Einsele; Armin Fiechter

• Publisher: Springer
• Year: 1981
• Tongue: English
• Weight: 537 KB
• Volume: 13
• Category: Article
• ISSN: 1432-0614
• DOI: 10.1007/bf00505334

✦ Synopsis

In this paper a new probe allowing the measurement of NAD(P)H-d_ependent culture fluorescence in a bioreactor is presented. This sterilizable probe can be inserted in every bioreactor using a standard fitting of 25 mm. Under well defined conditions high specificity and sensitivity as well as high stability are further advantages of this probe. Application examples are given to demonstrate the operation possibilities of this fluorescence probe. In batch growth the culture fluorescence can be used for on-line estimation of biomass concentration. Metabolic alterations due to substrate of oxygen deficiency can easily be detected by fluorometric measurements. In kinetic studies the fluorescence probe is of great use because of a very small time delay.