On-line measurement of hybridoma growth by culture fluorescence

✍ Scribed by G. MacMichael; W. B. Armiger; J. F. Lee; R. Mutharasan

• Publisher: Springer-Verlag
• Year: 1987
• Tongue: English
• Weight: 353 KB
• Volume: 1
• Category: Article
• ISSN: 0951-208X
• DOI: 10.1007/bf00155457

✦ Synopsis

Fluorescence measurement of viable hybridoma cell cultures provides a convenient method for monitoring the progress of a batch culture. It is shown that cell concentration changes as low as 35,000 cells/ml during initial stages of growth can be measured reliably. This sensitivity, however, decreases to 2 X 106 cells/ml at cell concentration greater than 2 X lo6 cells/ml. The culture fluorescence of hybridoma culture is a characteristic property of the cell and the medium used. Consequently, processes in which the medium composition and cell lines are invariant, a direct on-line estimate of viable cell count can be made using the method investigated in this paper.