✦ LIBER ✦

On-line high-performance liquid chromatography for the determination of cephalosporin C and by-products in complex fermentation broths

✍ Scribed by K. Holzhauer-Rieger; W. Zhou; K. Schügerl

Publisher: Elsevier Science
Year: 1990
Tongue: English
Weight: 421 KB
Volume: 499
Category: Article
ISSN: 1873-3778
DOI: 10.1016/s0021-9673(00)97005-5

No coin nor oath required. For personal study only.

✦ Synopsis

A fully automated high-performance liquid chromatographic system was developed for optimization of cephalosporin C production. Using a simple, isocratic, reversed-phase method, cephalosporin C, deacetylcephalosporin C, deacetoxycephalosporin C, penicillin N, methionine and its decomposition product 2-hydroxy-4-methylmercaptobutyric acid were determined.

The method worked reliably for 250 h and supplied information on the influence of many parameters of the process.

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