Determination by high-performance liquid chromatography of some compounds involved in the biosynthesis of penicillin and cephalosporin

✍ Scribed by John J. Usher; MaryAnn Lewis; Doris W. Hughes

• Publisher: Elsevier Science
• Year: 1985
• Tongue: English
• Weight: 357 KB
• Volume: 149
• Category: Article
• ISSN: 0003-2697
• DOI: 10.1016/0003-2697(85)90481-6

✦ Synopsis

A sensitive and convenient method for the simultaneous determination of several of the compounds involved in the biosynthesis of penicillin and cephalosporin is described. The method involves derivatization with a chiral fluorogen (o-phthaldialdehyde with an optically active thiol N-acetyl-L-cysteine), followed by high-performance liquid chromatography on a reverse-phase column as described by Aswad (D. W. Aswad, 1984, Anal. B&hem. 137,405-409). With an analysis time of 1 h it was possible to determine simultaneously most of the common L-amino acids, L-and D-a-aminoadipic acid, L-and D-valine, 6-@-cu-aminoadipyl)Lcysteine (AC), G-(L-cY-aminoadipyl)-L-cysteinyl-D-valine (ACV), glutathione, isopenicillin N, penicillin N, desacetoxycephalosporin C, desacetylcephalosporin C, and cephalosporin C. Using o-phthaldialdehyde alone it was possible to determine specifically the thiol-containing peptides AC, ACV, and glutathione. o 1985 Academic &ss, IIIC.