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On-line chemiluminescence detection for isoelectric focusing of heme proteins on microchips

✍ Scribed by Xiangyi Huang; Jicun Ren


Publisher
John Wiley and Sons
Year
2005
Tongue
English
Weight
182 KB
Volume
26
Category
Article
ISSN
0173-0835

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A novel on-line 2-D system was developed for peptide and protein mapping. The system combines capillary IEF (cIEF) with pressurized CEC (pCEC) using a micro-injection valve as the interface. Sample fractions, which were focused and separated in the firstdimension cIEF based on their differences in p