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Immunochemical detection of peptides and proteins on press-blots after direct tissue gel isoelectric focusing

✍ Scribed by Paul J. van der Sluis; Chris W. Pool; Arja A. Sluiter


Publisher
John Wiley and Sons
Year
1988
Tongue
English
Weight
890 KB
Volume
9
Category
Article
ISSN
0173-0835

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Application of cryostat sections directly onto mini ultrathin polyacrylamide isoelectric focusing gels allows an elution of proteins out of the sections into the gels under conventional focusing conditions. Protein bands representing alkaline phosphatases can easily be identified on nitrocellulose a