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On-capillary raman spectroscopy of nucleoside/borate complexes separated by isotachophoresis

✍ Scribed by Hongmin Li; Patrick A. Walker III; Michael D. Morris

Publisher: John Wiley and Sons
Year: 1998
Tongue: English
Weight: 150 KB
Volume: 10
Category: Article
ISSN: 1040-7685
DOI: 10.1002/(sici)1520-667x(1998)10:5<449::aid-mcs8>3.0.co;2-g

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✦ Synopsis

On-capillary normal Raman spectroscopy with 532 nm excitation is Ž . used as a detection method for capillary isotachophoresis ITP of the nucleosides: adenosine, cytidine, guanosine, and uridine. The compounds are separated as their borate complexes between leading electrolyte 0.1 M Cl y , pH 7.5, and terminating Ž . y electrolyte 0.1 M B OH , pH 10. The Raman spectra of the nucleosiderborate 4 complexes are presented. Additionally, Raman spectra are obtained from sepa-X Ž . rated adenosine and adenosine 5 -triphosphate ATP . Starting concentrations in the range 5.0 = 10 y6 ᎐5 = 10 y4 M are employed. Preconcentration factors of at least 10 3 are observed.

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