URA4, the gene coding for orotidine monophosphate decarboxylase (OMPdecase), has been cloned from the fission yeast by homologous complementation and restricted in anEscherichia coli-Schizosaccharomyces pombe (E. coli-S, pombe) replicative plasmid to a 1.76 kb HindlII fragment. This plasmid is maint
OMP decarboxylase—An enigma persists
✍ Scribed by Brian P. Callahan; Brian G. Miller
- Publisher
- Elsevier Science
- Year
- 2007
- Tongue
- English
- Weight
- 274 KB
- Volume
- 35
- Category
- Article
- ISSN
- 0045-2068
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The URA3 gene of Candida utilis encoding orotidine-5'-phosphate decarboxylase enzyme was isolated by complementation in Escherichia coli pyrF mutation. The deduced amino-acid sequence is highly similar to that of the Ura3 proteins from other yeast and fungal species. An extensive analysis of the fam
The yeast OMP decarboxylase gene has been cloned in the phage fd in both orientations to obtain pure DNA of each strand. With these probes the transcription of each strand of the yeast gene was measured in wild type yeast, either repressed or induced, in a yeast strain transformed by an hybrid plasm