Okadaic acid reversibly inhibits neurite outgrowth in embryonic dorsal root ganglion neurons

✍ Scribed by Giasson, Benoit I. ;Mushynski, Walter E.

• Book ID: 101258248
• Publisher: John Wiley and Sons
• Year: 1997
• Tongue: English
• Weight: 276 KB
• Volume: 32
• Category: Article
• ISSN: 0022-3034
• DOI: 10.1002/(sici)1097-4695(199702)32:2<193::aid-neu4>3.0.co;2-3

✦ Synopsis

The aim of this study was to assess used to eliminate proliferating cells did not alter the the effects of low concentrations of okadaic acid (OA) rate of neurite elongation. Since 1 nM OA does not on neurite outgrowth and cellular integrity in cultures suffice to inhibit neuronal protein phosphatase 2A of dissociated dorsal root ganglion (DRG) neurons.

fully, owing to the high concentration of this enzyme The complete and fully reversible arrest of neurite in neurons, we propose that the inhibitor is affecting outgrowth was achieved at 1 nM OA, thus ruling out a neuronal compartment that contains low levels of the involvement of protein phosphatase 1 in the obthe phosphatase. This putative compartment is likely served inhibitory effect. OA at 0.5 nM did not comto be located in neurites, which were shown to contain pletely block neurite outgrowth, although it reduced levels of protein phosphatase 2A that were two-to the rate of growth by about one third. Protein phosthreefold lower than in neuronal perikarya. ᭧ 1997 phorylation and the integrity of microtubules and neu-