ODMR of carotenoid and chlorophyll triplets in CP43 and CP47 complexes of spinach
โ Scribed by Donatella Carbonera; Giovanni Giacometti; Giancarlo Agostini; Alexander Angerhofer; Volker Aust
- Book ID
- 103032971
- Publisher
- Elsevier Science
- Year
- 1992
- Tongue
- English
- Weight
- 482 KB
- Volume
- 194
- Category
- Article
- ISSN
- 0009-2614
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โฆ Synopsis
Optically detected magnetic resonance (ODMR) of the light-harvesting complexes CP43 and CP47 of the spinach photosynthetic machinery revealed triplet states both from carotenoids and chlorophylls. The triplet state of the only carotenoid present in the complexes (p-carotene) is observed by ODMR using fluorescence detection (FDMR) in the main chlorophyll emission band in the 680 nm region, and absorption detection in the visible region. Chlorophyll triplet signals are also obtained by microwave sweeping in the chlorophyll emission and absorption regions. MIA spectra are obtained and discussed for both complexes.
๐ SIMILAR VOLUMES
Light-and heat-induced denaturation of the core-antenna complexes of Photosystem II, CP43 and CP47, purified from spinach, has been investigated using absorption, fluorescence and circular dichroism (CD) spectroscopy. Light is found to bring about considerable bleaching of chlorophyll a but no appar
Photosystem II from transplastomic plants of __Nicotiana tabacum__ with a hexahistidine tag at the N-terminal end of the PsbE subunit (ฮฑ-chain of the cytochrome b~559~) was purified according to the protocol of Fey et al. (BBA 12:1501โ1509, 2008). The protein sample was then subjected to two additio