✦ LIBER ✦

Isolation and crystallization of CP47, a Photosystem II chlorophyll binding protein. Degradation of CP47 upon dissociation from the core complex

✍ Scribed by E. Psylinakis; G. Fritzsch; D.F. Ghanotakis

Book ID: 110345861
Publisher: Springer
Year: 2002
Tongue: English
Weight: 327 KB
Volume: 72
Category: Article
ISSN: 0166-8595
DOI: 10.1023/a:1016136801753

No coin nor oath required. For personal study only.

📜 SIMILAR VOLUMES

Crystallization of the Photosystem II co

Crystallization of the Photosystem II core complex and its chlorophyll binding subunit CP43 from transplastomic plants ofNicotianatabacum

✍ Dario Piano; Sabah El Alaoui; Henryk J. Korza; Renata Filipek; Izabela Sabala; P 📂 Article 📅 2010 🏛 Springer 🌐 English ⚖ 344 KB

Photosystem II from transplastomic plants of __Nicotiana tabacum__ with a hexahistidine tag at the N-terminal end of the PsbE subunit (α-chain of the cytochrome b~559~) was purified according to the protocol of Fey et al. (BBA 12:1501–1509, 2008). The protein sample was then subjected to two additio

The role of CP 47 in the evolution of ox

The role of CP 47 in the evolution of oxygen and the binding of the extrinsic 33-kDa protein to the core complex of Photosystem II as determined by limited proteolysis

✍ Hidenori Hayashi; Yoko Fujimura; Prasanna S. Mohanty; Norio Murata 📂 Article 📅 1993 🏛 Springer 🌐 English ⚖ 737 KB

In order to identify the domain within Photosystem II complexes that functions in the evolution of oxygen, we performed limited proteolysis with lysylendopeptidase of the core complex of Photosystem II which had been depleted of the extrinsic 33-kDa protein (Mn-stabilizing protein). The cleavage sit

Selective extraction of CP 26 and CP 29

Selective extraction of CP 26 and CP 29 proteins without affecting the binding of the extrinsic proteins (33, 23 and 17 kDa) and the DCMU sensitivity of a Photosystem II core complex

✍ Ranjit K. Mishra; Demetrios F. Ghanotakis 📂 Article 📅 1994 🏛 Springer 🌐 English ⚖ 811 KB

A highly purified oxygen evolving Photosystem II core complex was isolated from PS II membranes solubilized with the non-ionic detergent n-octyl-/~-D-thioglucoside. The three extrinsic proteins (33, 23 and 17 kDa) were functionally bound to the PS II core complex. Selective extraction of the 22, 10