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Octadecylsilylated Porous Silica Rods as Separation Media for Reversed-Phase Liquid Chromatography

✍ Scribed by Minakuchi, Hiroyoshi; Nakanishi, Kazuki; Soga, Naohiro; Ishizuka, Norio; Tanaka, Nobuo

Book ID
120052834
Publisher
American Chemical Society
Year
1996
Tongue
English
Weight
188 KB
Volume
68
Category
Article
ISSN
0003-2700

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Fast protein separation by reversed-phas
Fast protein separation by reversed-phase high-performance liquid chromatography on octadecylsilyl-bonded nonporous silica gel: II. Improvement in recovery of hydrophobic proteins
✍ Hiroko Itoh; Noriyuki Nimura; Toshio Kinoshita; Norikazu Nagae; Mitsugu Nomura πŸ“‚ Article πŸ“… 1991 πŸ› Elsevier Science 🌐 English βš– 388 KB

Recovery of hydrophobic proteins from an RP-HPLC column was improved using a fast-separation RP-HPLC system operated at room temperature. Hydrophobic proteins such as ovalbumin could be adequately eluted from a nonporous octadecylsilyl (C18) spherical silica gel with a particle diameter of 20 micron