Occurrence, specific receptor sites and functional effects of endothelin in human cardiopulmonary tissue
✍ Scribed by A. Franco-Cereceda; R. Matran; A. Hemsén; A. Rudehill; E. Weitzberg; J.M. Lundberg
- Book ID
- 115883959
- Publisher
- Elsevier Science
- Year
- 1990
- Tongue
- English
- Weight
- 120 KB
- Volume
- 183
- Category
- Article
- ISSN
- 0014-2999
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Endothelin binding in human isolated lung membrane fractions revealed a single class of high affinity recognition sites with a Kd of 1.33 +/- 0.15 nM and a Bmax of 9.61 +/- 1.44 pmol/mg protein. Endothelin inhibited [125I]endothelin binding with a Ki of 1.90 +/- 0.15 nM whereas structurally unrelate
Specific endothelin-1 (ET1) binding sites have been demonstrated in membranes derived from normal (NP) and benign hyperplasic (BPH) human prostate using an lZ5I-ETl binding assay. '=I saturation experiments and Scatchard analysis demonstrated the existence of a homogeneous population of binding site