Occurrence of spermine in chromatin ofZea mays

✍ Scribed by Eiji Hirasawa; Yonezo Suzuki

• Publisher: Springer Netherlands
• Year: 1985
• Tongue: English
• Weight: 342 KB
• Volume: 3
• Category: Article
• ISSN: 0167-6903
• DOI: 10.1007/bf00117582

✦ Synopsis

Chromatin prepared from maize shoot tips using as extraction medium including quinacrine as an inhibitor of polyamine oxidase, contained 1.6 pmol spermidine /zg DNA -~ and 14.8pmol spermine /zg DNA -~ , respectively. This represented 0.1% spermidine and 3.7% spermine as compared with the content of those amines in the whole tissue. No putrescine was detectable in the chromatin preparation. When contamination of polyamines in the preparation was determined by the addition of labeled polyamines to the extraction medium, the ratio of the polyamines in the preparation to those in the extraction medium was 0.1% spermidine and 0.7% spermine, respectively. Spermine in the chromatin preparation was almost fully solubilized by a DNase-treatment, but spermidine was less easily solubilized. Most of the spermine associated with the chromation is chromatin-specific.