Observations on the biosynthesis of rat brain sphingolipids in vivo: Formation of fatty acid amides

✍ Scribed by Thomas P. Carter; Julian Kanfer

• Publisher: Elsevier Science
• Year: 1974
• Tongue: English
• Weight: 604 KB
• Volume: 13
• Category: Article
• ISSN: 0009-3084
• DOI: 10.1016/0009-3084(74)90008-5

✦ Synopsis

At early time intervals (1-10 min), intracerebrally injected [ 14C]-L-serine was incorporated into rat brain ceramide, cerebroside and sphingomyelin, as well as into serine-amide. Identification of the latter, most-highly labeled lipids, was based on TLC analyses employing an acidic, basic and neutral solvent system and on the liberation of free [14C]-serine during acid hydrolysis. The formation of [t4C]-serine-amide in vitro was solely dependent on the methodology employed for lipid purification, Preliminary evidence indicating the incorporation of [14C]-L" serine into both rat brain sphingosine-like and psychosine-like compounds in vivo is also presented. The significance of this latter observation is discussed.