High energy state quenching of chlorophyll fluorescence (qE) is inhibited by low concentrations of the inhibitor antimycin A in intact and osmotically shocked chloroplasts isolated from spinach and pea plants. This inhibition is independent of any effect upon ApH (as measured by 9-aminoacridine fluo
Observation and characterisation of a transient in the yield of chlorophyll fluorescence in intact spinach chloroplasts
β Scribed by K. A. Carver; P. Horton
- Publisher
- Springer
- Year
- 1987
- Tongue
- English
- Weight
- 428 KB
- Volume
- 11
- Category
- Article
- ISSN
- 0166-8595
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β¦ Synopsis
A transient in chlorophyll fluorescence, which is associated with a transient in 9-aminoacridine fluorescence and a perturbation in the rate of oxygen evolution, has been observed in intact spinach chloroplasts. The results indicate that changes in the redox state of Q are, at least partially, responsible for the transient in chlorophyll fluorescence. The size of the transient is highly dependent upon the concentration of inorganic phosphate and upon the pH of the medium. The properties of the transient are consistent with the suggestion that it reflects changes in the levels of stromal intermediates during induction.
π SIMILAR VOLUMES
We devised recently a method to trap intact isolated chloroplasts on a solid support consisting of membrane filters made of cellulose nitrate (Cerovi6 et al., 1987, Plant Physiol. 84, 1249-1251). The addition of alkaline phosphatase to the reaction medium enabled continuous photosynthesis by spinach
Simultaneous "ripples" (sudden changes in rate) in COs dependent 02 evolution and associated chlorophyU a fluorescence were followed in isolated, largely intact, spinach chloroplasts. These ripples could only be observed under conditions in which the supply of inorganic phosphate was limiting. This