We devised recently a method to trap intact isolated chloroplasts on a solid support consisting of membrane filters made of cellulose nitrate (Cerovi6 et al., 1987, Plant Physiol. 84, 1249-1251). The addition of alkaline phosphatase to the reaction medium enabled continuous photosynthesis by spinach
Photosynthetic oxygen evolution and chlorophyll fluorescence in intact isolated chloroplasts on a solid support: the influence of orthophosphate
✍ Scribed by Zoran G. Cerović; Zeljko Vučinić; David A. Walker
- Publisher
- Springer-Verlag
- Year
- 1991
- Tongue
- English
- Weight
- 595 KB
- Volume
- 184
- Category
- Article
- ISSN
- 0032-0935
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Simultaneous "ripples" (sudden changes in rate) in COs dependent 02 evolution and associated chlorophyU a fluorescence were followed in isolated, largely intact, spinach chloroplasts. These ripples could only be observed under conditions in which the supply of inorganic phosphate was limiting. This
The heat-sensitivity of photosynthetic oxygen evolution of thylakoids isolated from spinach increases by increasing the pH above neutral value. The temperature for inactivation (transition temperature) is lowered from about 45° C (pH 6.0-7.4) to 33°C (pH 8.5). Similar results are obtained with intac