Nucleotides in bivalves: Extraction and analysis by high-performance liquid chromatography (HPLC)
โ Scribed by J. Moal; J.R. Le Coz; J.F. Samain; J.Y. Daniel
- Book ID
- 113323042
- Publisher
- Elsevier Science
- Year
- 1989
- Tongue
- English
- Weight
- 798 KB
- Volume
- 93
- Category
- Article
- ISSN
- 1096-4959
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๐ SIMILAR VOLUMES
Procedures for the complete extraction and isocratic high-performance liquid chromatography analysis of cellular nucleoside di-and triphosphates are reported. Maximum nucleotide recovery from cultures of the ciliate Tetrahymenapyriformis is achieved if (a) cells are cooled to 4ยฐC prior to harvest; (
Nucleotides, nucleosides, and purine bases were extracted from human endomyocardial biopsies, freeze-clamped rat hearts, and porcine coronary sinus plasma. Perchloric acid extracts were neutralized with Freon-trioctylamine and analyzed at 250 nm by reverse-phase ion-pairing high-performance liquid c