Nucleotide sequence of theERG12gene ofSaccharomyces cerevisiaeencoding mevalonate kinase

✍ Scribed by A. Oulmouden; F. Karst

• Publisher: Springer-Verlag
• Year: 1991
• Tongue: English
• Weight: 612 KB
• Volume: 19
• Category: Article
• ISSN: 0172-8083
• DOI: 10.1007/bf00362081

✦ Synopsis

The nucleotide sequence of the ERG12 gene, encoding mevalonate kinase, from Saccharomyces cerevisiae is presented. The longest open reading frame may code for a protein containing 443 amino acids with a deduced relative molecular mass of 48 500. The analysis of the nucleotide sequence reveals a complete identity with the yeast gene RAR1, isolated elsewhere by complementation of a rat1 mutation involved in the stability of plasmids with weak ARS. In addition, we show that mevalonate kinase is not a rate-limiting enzyme; however its sensitivity to FFP could be a key regulatory mechanism in the sterol pathway of yeast.