𝔖 Bobbio Scriptorium
✦   LIBER   ✦

Nucleotide sequence of the recognition site of the B-specific restriction modification system in E. coli

✍ Scribed by Sommer, Reinhold ;Schaller, Heinz

Publisher
Springer
Year
1979
Tongue
English
Weight
850 KB
Volume
168
Category
Article
ISSN
0026-8925

No coin nor oath required. For personal study only.

✦ Synopsis

Two sB mutations in the genome of bacteriophage fd were located by sequence analysis in the fd sequence at positions 971 and 6341. Base changes at or close to these positions in phage M13 and in phage fl am 124 also correlate with a loss of sensitivity to B restriction. From the sequence homology between the sequences at the two sB sites the recognition signal for the E. coli B restriction/modification enzzyme is predicted to be: 5' TGA---8N---TGCT 3' 3' ACT---8N---ACGA 5'.

πŸ“œ SIMILAR VOLUMES

Restriction and modification in Bacillus
Restriction and modification in Bacillus subtilis: Expression of the cloned methyltransferase gene from B. subtilis phage SPR in E. coli and B. subtilis
✍ Montenegro, M. A. ;Pawlek, B. ;Behrens, B. ;Trautner, T. A. πŸ“‚ Article πŸ“… 1983 πŸ› Springer 🌐 English βš– 451 KB

Expression of the SPR methyltransferase gene from B. subtilis phage SPR cloned into lambda and SPP1 was studied by analyzing the sensitivity of the hybrid phage DNAs to restriction by the enzymes HaeIII, MspI, and HpaII. The following results were obtained: (1) The genes were expressed both in the h

Isolation and restriction mapping of pla
Isolation and restriction mapping of plasmids containing ribosomal DNA sequences from the rrn B cistron of E. coli
✍ Palmer, Margaret L. ;Raker, Mary Alice ;Kennedy, Poindexter J. ;Young, Jon W. ;B πŸ“‚ Article πŸ“… 1979 πŸ› Springer 🌐 English βš– 731 KB

Recombinant plasmids containing the entire 16S RNA gene from the rrn B cistron of E. coli inserted in Col E1 and pBR322 plasmid vectors have been constructed. These plasmids have been mapped using several restriction endonucleases as well as by DNA-RNA hybridization. These maps reveal previously und