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Nucleotide sequence of the gene for the peptidoglycan-associated lipoprotein of Escherichia coli K12

✍ Scribed by Robert CHEN; Ulf HENNING


Book ID
115124943
Publisher
John Wiley and Sons
Year
1987
Tongue
English
Weight
519 KB
Volume
163
Category
Article
ISSN
1432-1327

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The hemB gene of Escherichia coli K12, coding for porphobilinogen synthase (PBG-S; syn., 5-aminolevulinic acid dehydratase, ALA-D), was cloned following insertion of an EcoRI fragment of plasmid F'13 into the mobilizable vector pCR1. The hybrid plasmid carrying the hemB gene was able to complement a

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The hemA gene of Escherichia coli K12 was cloned by complementation of a hemA mutant of this organism. Subcloning of the initial 6.0 kb HindIII fragment allowed the isolation of a 1.5 kb NheI-AvaI fragment which retained the ability to complement the hemA mutant. DNA sequencing by the dideoxy chain