Nucleotide sequence of the EcoRII restriction endonuclease gene
β Scribed by Valery Kossykh; Alexander Repyk; Alexander Kaliman; Yaroslav Buryanov
- Book ID
- 119119782
- Publisher
- Elsevier Science
- Year
- 1989
- Tongue
- English
- Weight
- 483 KB
- Volume
- 1009
- Category
- Article
- ISSN
- 0167-4781
No coin nor oath required. For personal study only.
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## Abstract To elucidate the mechanism of action of restriction endonucleases __Mva__I and __Eco__RII a study was made of their interaction with a set of synthetic substrates in which the heterocyclic bases or the sugarβphosphate backbone had been modified; individual nucleotide residues had been r
The genes for restriction-modification system EcoRII have been cloned from plasmid N3 DNA using RSF2124 as a vector plasmid. The hybrid plasmids designated pFK321 and pFK322 contained a 5.8 megadaltons EcoRI--fragment derived from N3 DNA including the genes for restriction-modification system EcoRII