Molecular cloning of EcoRII endonuclease and methylase genes

✍ Scribed by Kosykh, Valeri G. ;Buryanov, Yaroslav I. ;Bayev, Alexander A.

• Book ID: 104729152
• Publisher: Springer
• Year: 1980
• Tongue: English
• Weight: 246 KB
• Volume: 178
• Category: Article
• ISSN: 0026-8925
• DOI: 10.1007/bf00337884

✦ Synopsis

The genes for restriction-modification system EcoRII have been cloned from plasmid N3 DNA using RSF2124 as a vector plasmid. The hybrid plasmids designated pFK321 and pFK322 contained a 5.8 megadaltons EcoRI--fragment derived from N3 DNA including the genes for restriction-modification system EcoRII and a gene for resistance to sulfanilamide.