Nucleosides and Nucleotides. 153. Effects of 2′-α- and 2′-β-Bromo-2′-deoxyadenosine on Oligonucleotide Hybridization and Nuclease Stability

✍ Scribed by Mitsutoshi Aoyagi; Yoshihito Ueno; Akira Ono; Akira Matsuda

• Publisher: Elsevier Science
• Year: 1996
• Tongue: English
• Weight: 286 KB
• Volume: 6
• Category: Article
• ISSN: 0960-894X
• DOI: 10.1016/s0960-894x(96)00277-6

✦ Synopsis

l-(2-Bromo-2-deoxy-l~-D-ribofuranosyl)adenine (1) and l-(2-bromo-2-deoxy-~-D-arabinofuranosyl)adenine (2) were incorporated into DNA pentadecamers. The oligonucleotides containing I destabilized DNA/DNA and DNA/RNA duplex formation. However, the oligonucleotides containing 2 slightly stabilized DNA/DNA duplex formation, although they decreased the stability of DNA/RNA duplexes. Furthermore. the oligonucleotide containing 2 was more resistant to snake venom phosphodiesterase than an unmodified oligonucleotide (dA)t~ and the oligonucleotide containing 1.