𝔖 Bobbio Scriptorium
✦   LIBER   ✦

Nucleoside diphosphate kinase at the cell surface of neoplastic human cells in culture

✍ Scribed by G. Ågren; J. Pontén; G. Ronquist; B. Westermark

Publisher
John Wiley and Sons
Year
1974
Tongue
English
Weight
931 KB
Volume
83
Category
Article
ISSN
0021-9541

No coin nor oath required. For personal study only.

✦ Synopsis

Abstract

Strong evidence is given that a nucleoside diphosphate kinase is present to some extent on the surface of intact neoplastic cells in culture. Experiments could be performed with cells cultured in a few plates to which an incubation medium was added. The cells were firmly attached to the supporting medium and remained viable during the incubation procedure.

Determinations of lactate dehydrogenase were carried out to rule out any possible contamination from the culturing medium as well as from the cell interior. From these analyses, a procedure was developed which easily removed the last traces of the culturing medium and which showed that there was no leakage of intracellular lactate dehydrogenase during the incubation procedure. There was a rather insignificant diffusion of nucleoside diphosphate kinase into the incubation medium. In common with other nucleoside diphosphate kinases, the glioma cell surface enzyme seemed to be nonspecific with regard to nucleotide substrates.

📜 SIMILAR VOLUMES

Surface glycoproteins of normal and neop
Surface glycoproteins of normal and neoplastic glia cells in culture
✍ Werner Lubitz; Bengt Westermark; Per A. Peterson 📂 Article 📅 1980 🏛 John Wiley and Sons 🌐 French ⚖ 559 KB

## Abstract Two normal and seven malignant human glia lines grown in vitro were labelled by lactoperoxidase catalysed iodination. The labelled cell surface glycoproteins were isolated by lectin affinity chromatography and compared by SDS gel electrophoresis. The glia and the glioma lines possess a

Formation of extracellular adenosine tri
Formation of extracellular adenosine triphosphate by normal and neoplastic human cells in culture
✍ G. Ågren; J. Ponten; G. Ronquist; B. Westermark 📂 Article 📅 1971 🏛 John Wiley and Sons 🌐 English ⚖ 418 KB

Normal and neoplastic human cells in culture were suspended under isotonic conditions and incubated for one minute with the substrates, including 82P-labelled inorganic phosphate, and cofactors of the glyceraldehyde-3-phosphate dehydrogenase and phosphoglycerate kinase reactions (complete medium), a

Insulin degrading enzyme is localized pr
Insulin degrading enzyme is localized predominantly at the cell surface of polarized and unpolarized human cerebrovascular endothelial cell cultures
✍ John A. Lynch; Ana M. George; Patricia B. Eisenhauer; Kelly Conn; Wenwu Gao; Isa 📂 Article 📅 2006 🏛 John Wiley and Sons 🌐 English ⚖ 243 KB 👁 1 views

## Abstract Insulin degrading enzyme (IDE) is expressed in the brain and may play an important role there in the degradation of the amyloid beta peptide (Aβ). Our results show that cultured human cerebrovascular endothelial cells (HCECs), a primary component of the blood–brain barrier, express IDE