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Nucleic acid sequence analysis of the precore region of hepatitis B virus from sera of southern African black adult carriers of the virus

✍ Scribed by A. Kramvis; S. Bukofzer; M. C. Kew; E. Song

Publisher
John Wiley and Sons
Year
1997
Tongue
English
Weight
281 KB
Volume
25
Category
Article
ISSN
0270-9139

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✦ Synopsis

HBV-DNA Quantitation in Serum. For quantitation of HBV DNA HBeAg is water soluble and enters the serum. 2 in serum, the Digene Hybrid Capture System (Digene Diagnostics,

The presence of HBeAg in the serum of hepatitis B surface Inc., Beltsville, MD), a chemiluminescent molecular hydridization antigen-positive patients is conventionally considered to be assay was used, according to the manufacturer's instructions. A slot blot hybridization assay, using the method of Scotto et al., 25 was run in parallel. Briefly, 50 mL of serum were diluted with 50 mL of 2 mol/ L NaCl, denatured with 100 mL 1 mol/L NaOH, and blotted onto Abbreviations: HBV, hepatitis B virus; HBeAg, hepatitis B e antigen; anti-HBe, hepatitis B e antigen antibody; PCR, polymerase chain reaction. QIABRANE nylon mebrane (Qiagen Inc., Hilden, Germany) using From the Medical Research Council/University of the Witwatersrand Molecular Hepatol-the Bio-Dot R SF microfiltration apparatus (BioLab Laboratories,

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