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Nucleic acid bases and derivatives: detection by Dns derivatization thin-layer chromatography

✍ Scribed by Gordon T. James; Allen B. Thach; Ellen Connole; James H. Austin; Robert Rinehart

Book ID
104144949
Publisher
Elsevier Science
Year
1980
Tongue
English
Weight
623 KB
Volume
195
Category
Article
ISSN
1873-3778

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Five nucleo derivatives have been chosen for quantitative in sitzc determination by reflectance spectroscopy after two-dimensional separation from a complex mixture by thin-layer chromatography on cellulose. The use of an internal standard to compensate for fluctuations in the chromatographic proced